Bacterial α-glucan phosphorylases

N-glycan degradation by bacterial glycoside phosphorylases 1 Role of glycoside-phosphorylases in mannose foraging by human gut bacteria*

In order to metabolise both dietary fibre constituent carbohydrates and host glycans lining the intestinal epithelium, gut bacteria produce a wide range of carbohydrate active enzymes, of which glycoside hydrolases are the main components. In this study, we describe the ability of phosphorylases to participate in the breakdown of human Nglycans, from an analysis of the substrate specificity of ...

Is the active form of pyridoxal-P in alpha-glucan phosphorylases a 5'-phosphate dianion?

The essential role of pyridoxal-5’-phosphate for the activity of all cY-glucan phosphorylases received strong support from recent studies with bacterial phosphorylases [ 11. The advantage of utilizing these enzymes for the study of the role of pyridoxal-P is obvious since they neither depend on allosteric effectors or covalent modification for the expression of activity [l-6]. The 5’-phosphate ...

Immunological differences of phosphorylases.

A number of reports in the literature indicate that there are immunological variations in enzymes from different sources (l-4), but these studies did not include a comparison of intracellular enzymes from different tissues of an animal. The experiments reported in this paper were undertaken to determine whether a particular enzyme (phosphorylase) differed immunologically in the tissues of a sin...

Irradiated β-glucan enhances immune response to bacterial infection through CD4 and CD8 T-lymphocytes

Background: &beta-glucans are glucose polymers with a variety of stimulatory effects on the immune system. The objective of this study was to evaluate the immune-enhancing activities of low molecular weight gamma irradiated &beta-glucan (I&beta-g) extracted from Pleurotus ostreatus in response to Pseudomonas aeruginosa infection in rats. Materials and Methods: &beta-glucan (&beta-g) powder was ...

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